10 20 30 40 50 60 70 ----:----|----:----|----:----|----:----|----:----|----:----|----:----| Consensus xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxlxxxxxxxxxxxxxxxxxxxxxxxx ==================================================================================================== EMERICELLA_NIDULANS ---------------------------------------------Vhlkt--lag-savfas-lataavl PENICILLIUM_SIMPLICISSIMUM ---------------------------------------------------------------------- THERMOASCUS_AURANTIACUS ---------------------------------------------Vrpti--llt-slllap-faaaspi 1TUX0_Thermoascus_aurantiacus ---------------------------------------------------------------------- Agaricus_bisporus ----------------------------------------------ryas--vea-sfdmap-mihlpgr Aspergillus_aculeatus ---------------------------------------------Vqika--aal-avlfas-nvlanpi Aspergillus_flavus ---------------------------------------------Vhlks--lag-illyts-lciass- Aspergillus_fumigatus ---------------------------------------------Vvlsk--lvs-silfvs-lvsag-v Aspergillus_niger ---------------------------------------------Vqika--aal-amlfas-hvlsepi Fusarium_oxysporum htfsvllalapvsalaqapiwgqcggngwtgattcasglkcekindwyyqcvpgsggsepqpsstqgggt Gibberella_zeae hflglvvafapaalaqsaiwgqcggtgwsgsttcqsglkcekindfyyqc--ipg-sdnggg-ttpdpgt Neurospora_crassa -kaslmml-------------------------------------Laplaamaaptsdnlepr------- Trichoderma_reesei -kanvilcl------------------------------------Laplvaalptetihldpelaalran ==================================================================================================== CLOSTRIDIUM_THERMOCELLUM -----------------------------------------------------------riitefpgqgd ==================================================================================================== Consensus xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxlxxxxxxxxxxxxxxxxxxxxxxxx 80 90 100 110 120 130 140 ----:----|----:----|----:----|----:----|----:----|----:----|----:----| Consensus xxxxxxxxxxqxxxxxxqaxxsVnxxxkxarGkxyfGtxxDqxxlsxnxxknxaIIqxdfgqVtpENsMK ==================================================================================================== EMERICELLA_NIDULANS --p-------R------Qs-aSLNdlfv-AaGKsYFGTcsDQalLq-Ns-QNeAIVasqFGvITPENSMK PENICILLIUM_SIMPLICISSIMUM -----------------QAsvSIDakfK-AhGKkYlGTigDQytLT-kntKNpAIIKaDFGQLTPENSMK THERMOASCUS_AURANTIACUS lee-------R------QAaqSVDqliK-ARGKvYFGvatDQnrLT-tg-KNaAIIQaNFGQVTPENSMK 1TUX0_Thermoascus_aurantiacus -----------------aAaqSVDqlid-ARGKvYFGvatDQnrLT-tg-KNaAIIQaDFGQVTPENSMK Agaricus_bisporus ssg-------R------pgqvlVltvnslpRGKkFFGaaaDQntIN-ia-aNqALLisDFGaVTPENSMK Aspergillus_aculeatus -ep-------R------QAsvSIDakfK-AhGKkYlGTigDQytLNkNa-KtpAIIKaDFGQLTPENSMK Aspergillus_flavus ----------Q------QApaSINnafv-AKGKkYFGTcaDQgtLS-Dg-tNsgIIKaDFGQLTPENSMK Aspergillus_fumigatus iee-------R------QAa-SINqaft-shGKkYFGTasDQalLq-ks-QNeAIVRkDFGQLTPENSMK Aspergillus_niger -ep-------R------QAsvSIDskfK-AhGKkYlGnigDQytLTkNs-KtpAVIKaDFGaLTPENSMK Fusarium_oxysporum pqptggn----------sggtgLDakfK-AKGKqYFGTeiDhyhLN-Nnpli-nIVKaqFGQVTcENSMK Gibberella_zeae --p-------spgngnadA-tgLDakiR-AKGKiYFGTeiDhyhLS-Nn-plinIVKkDFGQVTnENSMK Neurospora_crassa -----------------QAsqSIDalmK-AKGKlYFGactDQgrLT-Sg-KNavILQnNFGQVTPENSMK Trichoderma_reesei ltertadlwdR------QAsqSIDqliK-rKGKlYFGTatDRglLq-re-KNaAIIQaDlGQVTPENSMK ==================================================================================================== CLOSTRIDIUM_THERMOCELLUM vqtpnpsvtptqtpiptisgnaLrdyaE-ARGi-kiGTcvNypfyNnSdptynsILQrEFsmVvcENeMK ==================================================================================================== Consensus xxxxxxxxxxqxxxxxxqaxxsVnxxxkxarGkxyfGtxxDqxxlsxnxxknxaIIqxdfgqVtpENsMK 150 160 170 180 190 200 210 ----:----|----:----|----:----|----:----|----:----|----:----|----:----| Consensus WdaxEpnQgqfsFsgaDyLVnWAqxNgkxIRGHTlVWhSQlpxWVssisxDknsLtsVMknHIstLmxrY ==================================================================================================== EMERICELLA_NIDULANS WDAlEPSQGnFgWSGADYLVDYAtqHnKkVRGHTLVWHSQLPsWVSSIg-DaNTLrSVMtNHINeVVgRY PENICILLIUM_SIMPLICISSIMUM WDAtEPNRGQFTFSGsDYLVNFAQsNGKlIRGHTLVWHSQLPgWVSSIT-DKNTLiSVLKNHITTVMtRY THERMOASCUS_AURANTIACUS WDAtEPSQGnFNFaGADYLVNWAQqNGKlIRGHTLVWHSQLPsWVSSIT-DKNTLTNVMKNHITTLMtRY 1TUX0_Thermoascus_aurantiacus WDAtEPSQGnFNFaGADYLVNWAQqNGKlIRGHTLVWHSQLPsWVvSIT-DKNTLTNVMKNHITTIMtRY Agaricus_bisporus WDAtEPNRGQFNFgGADFLVNWAtsHGKmIRGHTfVWHSQLPgWVSSIN-DRtTLTSVIQNHISTLggRY Aspergillus_aculeatus WDAtEPNRGQFSFSGsDYLVNFAQsNGKlIRGHTLVWHSQLPsWVqSIy-DKgTLiqVMQNHIaTVMqRY Aspergillus_flavus WDAtEPSQGKFSFSGADYLVNYAatNnKlIRGHTLVWHSQLPsWVqgIT-DKNTLTSVLKNHITTVMnRY Aspergillus_fumigatus WDAtEPSQGRFNFaGADFLVNYAKqNGKkVRGHTLVWHSQLPsWVSaIS-DKNTLTSVLKNHITTVMtRY Aspergillus_niger WDAtEPSRGQFSFSGsDYLVNFAQsNnKlIRGHTLVWHSQLPsWVqaIT-DKNTLieVMKNHITTVMqhY Fusarium_oxysporum WDAiEPSRnsFTFSnADkVVDFAtqNGKlIRGHTLLWHSQLPqWVqNIN-DRSTLTaVIENHVkTMVtRY Gibberella_zeae WDAiEPSRGQFTFSnADkVVDFAQaNGKkIRGHTLLWySQLPqWVkNIr-DRaTMTSVIENHVkTVVtRY Neurospora_crassa WDsiQPqRGQYNFgqADYLVNWAtaNnKtIRGHTfLWHSQLagWVNNIr-DKNqLTTVLQeHITTVMtRY Trichoderma_reesei WqslEnNQGQlNWgdADYLVNFAQqNGKsIRGHTLIWHSQLPaWVNNIN-NaDTLrqVIRtHVSTVVgRY ==================================================================================================== CLOSTRIDIUM_THERMOCELLUM FDAlQPrQnvFdFSkgDqLLaFAErNGmqMRGHTLIWHNQnPsWLTNgNwNRDSLlaVMKNHITTVMthY ==================================================================================================== Consensus WdaxEpnQgqfsFsgaDyLVnWAqxNgkxIRGHTlVWhSQlpxWVssisxDknsLtsVMknHIstLmxrY 220 230 240 250 260 270 280 ----:----|----:----|----:----|----:----|----:----|----:----|----:----| Consensus kGKIxaWDVvNEifnEdGxslRnTvFxxVIGEdfIxiAFQxARaaDPnAkLYiNDYNLDsanyxKxxxsx ==================================================================================================== EMERICELLA_NIDULANS KGKImhWDVVNEIFNEDG-TfRNSVFynLLGEDFVrIAFEtARAADPDAKLYINDYNLDSASYaK-t-q- PENICILLIUM_SIMPLICISSIMUM KGKIyAWDVLNEIFNEDG-SLRNSVFynVIGEDYVrIAFEtARsvDPNAKLYINDYNLDSAgYsK-v-N- THERMOASCUS_AURANTIACUS KGKIrAWDVVNEaFNEDG-SLRqTVFlnVIGEDYIpIAFQtARAADPNAKLYINDYNLDSASYpK-t-q- 1TUX0_Thermoascus_aurantiacus iGKIrAWDVVNEaFNEDG-SLRqTVFnnVIGEDYIpIAFRtARAADPNAKLYINDYNLDSASkpK-t-S- Agaricus_bisporus RGKIyAWDVcNEIFNEDG-SIRqSVFsnVLGEsFVtIAFQaARsADPNAKLYINDYNLDSnN-aK-v-q- Aspergillus_aculeatus KGKVyAWDVVNEIFNEDG-SLRqShFynVIGEDYVrIAFEtARAvDPNAKLYINDYNLDSASYpK-l-T- Aspergillus_flavus KGKVyAWDVVNEIFNEDG-TLRSSVFynVLGEDFVrIAFEtARAADPqAKLYINDYNLDSANYgK-t-T- Aspergillus_fumigatus KGQIyAWDVVNEIFNEDG-SLRDSVFsrVLGEDFVrIAFEtARsvDPSAKLYINDYNLDSASYgK-t-q- Aspergillus_niger KGKIyAWDVVNEIFNEDG-SLRDSVFykVIGDDYVrIAFEtARAADPNAKLYINDYNLDSASYpK-l-a- Fusarium_oxysporum KGKIlqWDVVNEIFaEDG-NLRDSVFsrVLGEDFVgIAFRaARAADPaAKLYINDYNLDksDYaK-l-Tr Gibberella_zeae KGKIlhWDVVNEIFaEDG-NMRNSeFyqVLGEDFVgIAFRaARAADPaAKLYINDYNLDiANYaKvt-r- Neurospora_crassa KGKIygYDVaNEIFNEDG-SLRSSIFyqVLGEDmVsIAFKaARAADPNAKLYINDYNLDSpNYaK-vtT- Trichoderma_reesei KGKIrAWDVVNEIFNEDG-TLRSSVFsrLLGEEFVsIAFRaARdADPSARLYINDYNLDrANYgK-v-N- ==================================================================================================== CLOSTRIDIUM_THERMOCELLUM KGKIveWDVaNEcmDDsGngLRSSIWrnVIGQDYLdyAFRyAReADPDAlLFyNDYNIEdl-gpK-s-N- ==================================================================================================== Consensus kGKIxaWDVvNEifnEdGxslRnTvFxxVIGEdfIxiAFQxARaaDPnAkLYiNDYNLDsanyxKxxxsx 290 300 310 320 330 340 350 ----:----|----:----|----:----|----:----|----:----|----:----|----:----| Consensus gIisxvkkwlaxgvpidgigsqxhlxxxxxgxxxxxgxxxxxxvxxxaLxxlAxxgxxeVaiTELDVxxx ==================================================================================================== EMERICELLA_NIDULANS aMaSyVKKWLAeGVPIDGIGSQaHys-sshw----ss----tea-agALssLAntGvsEVAITELDIa-- PENICILLIUM_SIMPLICISSIMUM GMVShVKKWLAaGIPIDGIGSQtHLg-a--G----aG----saV-agALnaLAsaGtkEIAITELDIa-- THERMOASCUS_AURANTIACUS aIVNrVKKWrAaGVPIDGIGSQtHLs-a--G----qG----asV-lqALplLAsaGtpEVAITELDVa-- 1TUX0_Thermoascus_aurantiacus aIVkrVKKWrAaGVPIDGIGSQtHLs-a--G----qG----asI-daALpnLAsaGtpEVAITELDIa-- Agaricus_bisporus GMValVKRqnAnGriIDGIGTQmHLg-p--G----gG----sga-qaAItaLAgaGt-ELAITELDIq-- Aspergillus_aculeatus GLVNhVKKWVAaGVPIDGIGSQtHLs-a--G----aG----aaV-sgALnaLAgaGtkEVAITELDIa-- Aspergillus_flavus GLaNhVKKWIAqGIPIDGIGSQtHLs-a--G----gs----sgV-kgALntLAasGvsEVAITELDIa-- Aspergillus_fumigatus GMalkptlvrvlr-PaskdkSpwfsc-l--r----na----dpp-ctALtaLAssGvsEVAITELDIa-- Aspergillus_niger GMVShVKKWIeaGIPIDGIGSQtHLs-a--G----gG----agI-sgALnaLAgaGtkEIAVTELDIa-- Fusarium_oxysporum GMVahVnKWIAaGIPIDGIGSQgHLa-a--p----sGwnpasgV-paALraLAasdakEIAITELDIs-- Gibberella_zeae GMVdhVnKWVsqGIPIDGIGSQaHLakpggw----np----asgfpaALkvLAganvkEVAITELDId-- Neurospora_crassa GMIqhVnKWVAaGVPIDGIGTQgHIn-a--G----gG----snL-aaAIkaLAsanvkEVAVTELDIq-- Trichoderma_reesei GLkTyVsKWIsqGVPIDGIGSQsHLs-g--G----gG----sgt-lgALqqLAtvpvtELAITELDIq-- ==================================================================================================== CLOSTRIDIUM_THERMOCELLUM aVfNmIKsmkerGVPIDGVGfQcHfi-n--Gmspeyl----asI-dqnIkryAeiGv-iVsfTEIDIrip ==================================================================================================== Consensus gIisxvkkwlaxgvpidgigsqxhlxxxxxgxxxxxgxxxxxxvxxxaLxxlAxxgxxeVaiTELDVxxx 360 370 380 390 400 410 420 ----:----|----:----|----:----|----:----|----:----|----:----|----:----| Consensus xxxxxxxxxgasssDYvxVvxaCLnxpkCvgitvWGixDxdSWrxxsxxxxxSPLLFDxNFxPKxAYnaI ==================================================================================================== EMERICELLA_NIDULANS ---------GAaSSDYLnLLnACLNeqKCVGITVWGVsDkDSWRasd-----SPLLFDgNYqPKdAYNAI PENICILLIUM_SIMPLICISSIMUM ---------GASSTDYVnVVnACLNqaKCVGITVWGVaDpDSWRssS-----SPLLFDgNYnPKaAYNAI THERMOASCUS_AURANTIACUS ---------GASSTDYVnVVnACLNvssCVGITVWGVaDpDSWRasT-----TPLLFDgNFnPKpAYNAI 1TUX0_Thermoascus_aurantiacus ---------GATSTDYVdVVnACLDvdsCIGITVWGVaDpDSWRasT-----TPLLFDgNFnPKpAYNAI Agaricus_bisporus ---------nASSSDYVaVVnACLNqPaCVsITtWGVaDiNSWRsgS-----SPLLFDnNYrPKaAYNAV Aspergillus_aculeatus ---------GASSTDYVnVVkACLNqPKCVGITVWGVaDpDSWRssS-----SPLLFDsNYnPKaAYtAI Aspergillus_flavus ---------GASSNDYVnVVeACLevsKCVGITVWGVsDkNSWRsae-----SPLLFDgNYqPKsAYNAI Aspergillus_fumigatus ---------GASSqDYVnVVkACLDvPKCVGITVWGVsDrDSWRsgS-----SPLLFDsNYqPKaAYNAI Aspergillus_niger ---------GASSTDYVeVVeACLDqPKCIGITVWGVaDpDSWRssS-----TPLLFDsNYnPKpAYtAI Fusarium_oxysporum ---------GASaNDYLtVMnACLavPKCVGITVWGVsDkDSWRpge-----NPLLYDsNYqPKaAFNAL Gibberella_zeae ---------GAaaNDYVtVVnsCLttPKCVGITVWGVsDkDSWRseS-----NPLLFDrNYqPKaAYtAV Neurospora_crassa ---------GnNagDYatItkgCLSePKCVGITVWGVrDsDSWRpng-----NPLLFDaNWnPKaAYNsI Trichoderma_reesei ---------GApTTDYtqVVqACLSvsKCVGITVWGIsDkDSWRasT-----NPLLFDaNFnPKpAYNsI ==================================================================================================== CLOSTRIDIUM_THERMOCELLUM qsenpatafqvqaNNYkeLMkiCLanPnCntfvMWGftDkyTWipgTfpgygNPLIYDsNYnPKpAYNAI ==================================================================================================== Consensus xxxxxxxxxgasssDYvxVvxaCLnxpkCvgitvWGixDxdSWrxxsxxxxxSPLLFDxNFxPKxAYnaI 430 440 450 460 470 480 490 ----:----|----:----|----:----|----:----|----:----|----:----|----:----| Consensus vnaL ================================== EMERICELLA_NIDULANS VNAL PENICILLIUM_SIMPLICISSIMUM aNAL THERMOASCUS_AURANTIACUS VqnL 1TUX0_Thermoascus_aurantiacus VqlL Agaricus_bisporus INAL Aspergillus_aculeatus aNAL Aspergillus_flavus LNAL Aspergillus_fumigatus IaAL Aspergillus_niger aNAL Fusarium_oxysporum VNAL Gibberella_zeae sNAL Neurospora_crassa VqAL Trichoderma_reesei VgiL ================================== CLOSTRIDIUM_THERMOCELLUM keAL ================================== Consensus vnaL